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Groupleader: Felix Wieland
Felix Wieland
Vesicular Transport, Protein-Lipid Interactions
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Research
Our research interests comprise two converging fields:
i) Molecular mechanisms of coated vesicle formation,
and
ii) Specificity and structural basis for protein-lipid interactions within the bilayer,
in collaboration with Prof. Britta Brügger
i) Molecular mechanisms of coated vesicle formation,
and
ii) Specificity and structural basis for protein-lipid interactions within the bilayer,
in collaboration with Prof. Britta Brügger
i) Molecular mechanisms of coated vesicle formation
In the eukaryotic cell, vesicular transport represents the basic mechanism for i) maintaining the homeostasis of the endomembrane system, ii) biosynthetic transport of newly synthesized proteins and lipids, and iii) the uptake and intracellular transport of exogenous macromolecules. Three classes of coated vesicles are well established to mediate transport in the exo- and endocytic pathway: COPII vesicles for ER export, COPI vesicles for retrograde Golgi-ER and bidirectional intra-Golgi transport, and clathrin-coated vesicles operating in the late secretory and endocytic pathway. Coat components are involved in multiple tasks such as cargo selection, curvature formation at the donor membrane, vesicle fission and initiation of uncoating.
We are interested in the molecular mechanisms underlying intracellular transport by COPI coated vesicles. In contrast to COPII and clathrin coats, the heptameric large COPI coat component coatomer is recruited en bloc to the membrane, so that both the inner and outer shell of the vesicle are formed at the same time. Recently, the two coatomer subunits γ-COP and ζ-COP were found to exist in two isoforms. Each isoform is, like all other subunits, present in coatomer as one copy, resulting in four possible different heptameric protein complexes. We found that these coatomer isoforms localize differentially within the Golgi of mammalian cells, suggesting different sites of budding for each of them.
In our view, the formation of a COPI transport vesicle involves the following minimal set of components: donor membranes with transmembrane proteins acting as coat and/or cargo receptors (e.g. members of the p24 family), cytosolic Arf1, cytosolic coatomer and auxiliary enzymes that serve as nucleotide exchange factors for activation on the membrane of Arf1 (GBF1) and GTPase activating proteins for the activation of GTP hydrolysis by Arf1 (Arf GAPs).
We are studying the cooperation of these proteins in the formation of the COPI coat and in the scission of COPI coated buds with a wide range of methods in Biochemistry, Molecular Biology, Cell Biology and Biophysics.
In collaboration with John Briggs’ and Irmi Sinning’s groups we are interested in the structure of the coat protein coatomer as a monomeric, soluble complex, as well as in its membrane bound form as a coating network.
Our present view of the molecular mechanisms underlying the process of budding are depicted in the figure above.
We are interested in the molecular mechanisms underlying intracellular transport by COPI coated vesicles. In contrast to COPII and clathrin coats, the heptameric large COPI coat component coatomer is recruited en bloc to the membrane, so that both the inner and outer shell of the vesicle are formed at the same time. Recently, the two coatomer subunits γ-COP and ζ-COP were found to exist in two isoforms. Each isoform is, like all other subunits, present in coatomer as one copy, resulting in four possible different heptameric protein complexes. We found that these coatomer isoforms localize differentially within the Golgi of mammalian cells, suggesting different sites of budding for each of them.
In our view, the formation of a COPI transport vesicle involves the following minimal set of components: donor membranes with transmembrane proteins acting as coat and/or cargo receptors (e.g. members of the p24 family), cytosolic Arf1, cytosolic coatomer and auxiliary enzymes that serve as nucleotide exchange factors for activation on the membrane of Arf1 (GBF1) and GTPase activating proteins for the activation of GTP hydrolysis by Arf1 (Arf GAPs).
We are studying the cooperation of these proteins in the formation of the COPI coat and in the scission of COPI coated buds with a wide range of methods in Biochemistry, Molecular Biology, Cell Biology and Biophysics.
In collaboration with John Briggs’ and Irmi Sinning’s groups we are interested in the structure of the coat protein coatomer as a monomeric, soluble complex, as well as in its membrane bound form as a coating network.
Our present view of the molecular mechanisms underlying the process of budding are depicted in the figure above.
Collaborations with:
James E. Rothman, Yale
Graham Warren, Vienna,Rainer Pepperkok, EMBL Heidelberg
John Briggs, EMBL Heidelberg
Irmi Sinning, BZH, Heidelberg
Selected publications on Molecular mechanisms of coated vesicle formation:
F. Wieland, M. Gleason, T. Serafini, J. Rothman (1987) The Rate of Bulk Flow from the Endoplasmic Reticulum to the Cell Surface. Cell 50, 289-300.
M. R. Block, B. S. Glick, C. A. Wilcox, F. Wieland, and J. Rothman (1988) Purification of an N-Ethylmaleimide-Sensitive Protein Catalyzing Vesicular Transport. Proc. Natl. Acad. Sci.U.S.A. 85, 7852-7856.
A. Karrenbauer, D. Jeckel. W. Just, R. Birk, R. R. Schmidt, J. E. Rothman, and F. Wieland (1990) The Rate of Bulk Flow from the Golgi to the Plasma Membrane. Cell 63, 259-267.
T. Serafini, G. Stenbeck, A. Brecht, F. Lottspeich, L. Orci, J. E. Rothman, and F. Wieland (1991) A Coat Subunit of Golgi-Derived non-Clathrin Coated Vesicles with Homology to the Clathrin Coated Vesicle Coat Protein b-Adaptin. Nature 349, 215-220.
G. Stenbeck, C. Harter, A. Brecht, D. Herrmann, F. Lottspeich, L. Orci, and F. T. Wieland (1993) b'-COP, a Novel Subunit of Coatomer. EMBO J. 12, 2841-2845.
J. E. Rothman and F. T. Wieland (1996) Protein sorting by transport vesicles. Science 272, 227-234.
K. Sohn, L. Orci, M. Ravazzola, M. Amherdt, M. Bremser, F. Lottspeich, K. Fiedler, J. B. Helms, and F. Wieland (1996) A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding. J. Cell Biol. 135, 1238-1248.
C. Reinhard, C. Harter, M. Bremser, B. Brügger, K. Sohn. B. Helms, and F. Wieland (1999) Receptor induced polymerisation of coatomer. Proc. Natl. Acad. Sci. U.S.A. 96, 1224-1228.
M. Bremser, W. Nickel, M. Schweikert, M. Ravazzola, M. Amherd, C.A. Hughes, T.H. Söllner, J. E. Rothman, and F. T. Wieland (1999) Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors. Cell 96, 495-506.
D. Gommel, A. R. Memon, A. Heiss, F. Lottspeich, J. Pfannstiel, J. Lechner, C. Reinhard, J. B. Helms, W. Nickel, F. T. Wieland (2001) Recruitment to Golgi Membranes of ADP-Ribosylation Factor is mediated by the cytoplasmic domain of p23, EMBO J. 20, 6751-6760.
C. Reinhard, M. Schweikert, F.T. Wieland, W. Nickel (2003) Functional reconstitution of COPI coat assembly and disassembly using chemically defined components. Proc. Natl. Acad. Sci. USA 100, 8253-8257.
D. Wegmann, P. Hess, C. Baier, F. T. Wieland and C. Reinhard (2004). Novel isotypic g/z-subunits reveal three coatomer complexes in mammals. Mol. Cell. Biol. 24, 1070-1080.
J. Moelleken, J. Malsam, M.J. Betts, A. Movafeghi, I. Reckmann, I. Meissner, A. Hellwig, R.B. Russell, T. Söllner, B. Brügger and F. Wieland (2007): Differential localization of coatomer complex isoforms within the Golgi apparatus. Proc. Natl. Acad. Sci. USA, 104: 4425-4430.
R. Beck, Z. Sun, F. Adolf, C. Rutz, J. Bassler, K. Wild, I. Sinning, E. Hurt, B. Brügger, J. Béthune*, and F. Wieland* (2008) Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proc.Natl.Acad.Sci.USA, 105:11731-6.
R. Beck, S. Prinz, P. Diestelkötter-Bachert, S. Röhling, F. Adolf, K. Hoehner, S. Welsch, P. Ronchi, B. Brügger, J.A. Briggs, and F. Wieland (2011) Coatomer and dimeric ADP ribosylation factor 1 (Arf1) promote distinct steps in membrane scission. J Cell Biol. 194(5):765-77.
M. Faini, S. Prinz, R. Beck, M. Schorb, J.D. Riches, K. Bacia, B. Brügger, F.T. Wieland & J.A.G. Briggs (2012). The Structures of COPI-Coated Vesicles Reveal Alternate Coatomer Conformations and Interactions. Science. Epub: 10.1126/science.1221443
M. R. Block, B. S. Glick, C. A. Wilcox, F. Wieland, and J. Rothman (1988) Purification of an N-Ethylmaleimide-Sensitive Protein Catalyzing Vesicular Transport. Proc. Natl. Acad. Sci.U.S.A. 85, 7852-7856.
A. Karrenbauer, D. Jeckel. W. Just, R. Birk, R. R. Schmidt, J. E. Rothman, and F. Wieland (1990) The Rate of Bulk Flow from the Golgi to the Plasma Membrane. Cell 63, 259-267.
T. Serafini, G. Stenbeck, A. Brecht, F. Lottspeich, L. Orci, J. E. Rothman, and F. Wieland (1991) A Coat Subunit of Golgi-Derived non-Clathrin Coated Vesicles with Homology to the Clathrin Coated Vesicle Coat Protein b-Adaptin. Nature 349, 215-220.
G. Stenbeck, C. Harter, A. Brecht, D. Herrmann, F. Lottspeich, L. Orci, and F. T. Wieland (1993) b'-COP, a Novel Subunit of Coatomer. EMBO J. 12, 2841-2845.
J. E. Rothman and F. T. Wieland (1996) Protein sorting by transport vesicles. Science 272, 227-234.
K. Sohn, L. Orci, M. Ravazzola, M. Amherdt, M. Bremser, F. Lottspeich, K. Fiedler, J. B. Helms, and F. Wieland (1996) A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding. J. Cell Biol. 135, 1238-1248.
C. Reinhard, C. Harter, M. Bremser, B. Brügger, K. Sohn. B. Helms, and F. Wieland (1999) Receptor induced polymerisation of coatomer. Proc. Natl. Acad. Sci. U.S.A. 96, 1224-1228.
M. Bremser, W. Nickel, M. Schweikert, M. Ravazzola, M. Amherd, C.A. Hughes, T.H. Söllner, J. E. Rothman, and F. T. Wieland (1999) Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors. Cell 96, 495-506.
D. Gommel, A. R. Memon, A. Heiss, F. Lottspeich, J. Pfannstiel, J. Lechner, C. Reinhard, J. B. Helms, W. Nickel, F. T. Wieland (2001) Recruitment to Golgi Membranes of ADP-Ribosylation Factor is mediated by the cytoplasmic domain of p23, EMBO J. 20, 6751-6760.
C. Reinhard, M. Schweikert, F.T. Wieland, W. Nickel (2003) Functional reconstitution of COPI coat assembly and disassembly using chemically defined components. Proc. Natl. Acad. Sci. USA 100, 8253-8257.
D. Wegmann, P. Hess, C. Baier, F. T. Wieland and C. Reinhard (2004). Novel isotypic g/z-subunits reveal three coatomer complexes in mammals. Mol. Cell. Biol. 24, 1070-1080.
J. Moelleken, J. Malsam, M.J. Betts, A. Movafeghi, I. Reckmann, I. Meissner, A. Hellwig, R.B. Russell, T. Söllner, B. Brügger and F. Wieland (2007): Differential localization of coatomer complex isoforms within the Golgi apparatus. Proc. Natl. Acad. Sci. USA, 104: 4425-4430.
R. Beck, Z. Sun, F. Adolf, C. Rutz, J. Bassler, K. Wild, I. Sinning, E. Hurt, B. Brügger, J. Béthune*, and F. Wieland* (2008) Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proc.Natl.Acad.Sci.USA, 105:11731-6.
R. Beck, S. Prinz, P. Diestelkötter-Bachert, S. Röhling, F. Adolf, K. Hoehner, S. Welsch, P. Ronchi, B. Brügger, J.A. Briggs, and F. Wieland (2011) Coatomer and dimeric ADP ribosylation factor 1 (Arf1) promote distinct steps in membrane scission. J Cell Biol. 194(5):765-77.
M. Faini, S. Prinz, R. Beck, M. Schorb, J.D. Riches, K. Bacia, B. Brügger, F.T. Wieland & J.A.G. Briggs (2012). The Structures of COPI-Coated Vesicles Reveal Alternate Coatomer Conformations and Interactions. Science. Epub: 10.1126/science.1221443
ii) Specificity and structural basis for protein-lipid interactions
within the bilayer
Functioning and processing of membrane proteins critically depend on the way their transmembrane domains (TMDs) are embedded in the membrane. Sphingolipids are structural components of membranes and can also act as intracellular second messengers. Not much is known of sphingolipids binding to TMDs of proteins within the hydrophobic bilayer, and how this could affect protein function. To our surprise, we found a direct and highly specific interaction of exclusively one sphingomyelin species, SM 18, with the TMD of the COPI machinery protein p24. Strikingly, the interaction depends on both the head group and the backbone of the sphingolipid, and on a signature (molecular recognition determining structure (MRDS) VXXTLXXIY within the TMD. Molecular dynamics simulations show a close interaction of SM 18 with the TMD (see figure on the left). We suggest a role of SM 18 in regulating the equilibrium between an inactive monomeric and an active oligomeric state of the p24 protein, which in turn regulates COPI-dependent transport. Bioinformatic analyses predict that the MRDS represents a conserved sphingolipid-binding cavity that exists in a variety of mammalian membrane proteins. Thus, in addition to a function as second messengers, sphingolipids can act as cofactors to regulate the function of transmembrane proteins. Our discovery of an unprecedented specificity of interaction of a TMD with an individual sphingolipid species adds to our understanding of why biological membranes are assembled from such a large variety of different lipids.
We have defined a number of trans-membrane proteins as candidates for specific interaction with sphingolipids, and in the future will investigate structure and function of such lipid/trans membrane complexes.
We have defined a number of trans-membrane proteins as candidates for specific interaction with sphingolipids, and in the future will investigate structure and function of such lipid/trans membrane complexes.
Collaborations with: Gunnar von Heijne, Stockholm,
Erik Lindahl, Stockholm,
Patrick Elofsson, Stockholm,
Christoph Thiele, Bonn,
Rainer Pepperkok, EMBL, Heidelberg
Vivek Malhotra, Barcelona
Selected publications on Specificity and structural basis for protein-lipid interactions within the bilayer:
A. Karrenbauer, D. Jeckel. W. Just, R. Birk, R. R. Schmidt, J. E. Rothman, and F. Wieland (1991) The Rate of Bulk Flow from the Golgi to the Plasma Membrane. Cell 63, 259-267.
D. Jeckel, A. Karrenbauer, K. N. J. Burger, G. van Meer, and F. Wieland (1992). Glucosylceramide is synthesized at the cytosolic surface of various Golgi subfractions. J. Cell Biol. 117, 259-267.
A. Brüning, A. Karrenbauer, E. Schnabel, and F. Wieland (1992) Brefeldin A-Induced Increase of Sphingomyelin Synthesis. J. Biol. Chem. 267, 5052-5055.
M. Murata, J. Peränen, R. Schreiner, F. Wieland, T. V. Kurzchalia, and K. Simons (1995) VIP21/caveolin is a cholesterol-binding protein. Proc. Natl. Acad. Sci. U.S.A. 92, 10339-10343.
H. Lannert, K. Gorgas, I. Meißner, F. Wieland, and D. Jeckel (1998) Lactosylceramide and subsequent glycosphingolipids are synthesized in the late Golgi. J. Biol. Chem. 273, 2939-2946.
B. Brügger, R. Sandhoff, S. Wegehingel, K. Gorgas, J. Malsam, B. Helms, W.-D. Lehmann, W. Nickel, and F. Wieland (2000) Evidence for Segregation of sphingomyelin and cholesterol during formation of COPI-coated vesicles. J. Cell Biol. 151, 507-517.
I. Gkantiragas, B. Brügger, E. Stüven, D. Kaloyanova, X. Li, K. Löhr, F. Lottspeich, F. T. Wieland, and B. Helms (2001) Sphingomyelin-enriched microdomains at the Golgi complex. Mol. Biol. Cell 12, 1819-1833.
P. Haberkant, O. Schmitt, F.-X. Contreras, C. Thiele, K. Hanada, H. Sprong, C. Reinhard, F. T. Wieland, B. Brügger (2008). Protein-sphingolipid interactions within cellular membranes. J. Lipid Res. 49(1):251-62.
F.X. Contreras, A.M. Ernst, F. Wieland and B. Brügger (2011). Specificity of intramembrane protein-lipid interactions. Cold Spring Harb Perspect Biol. Jun 1;3(6)
F.-X. Contreras, A.M. Ernst, P. Haberkant, P. Björkholm, E. Lindahl, B. Gönen, C. Tischer, A. Elofsson, G. von Heijne, C. Thiele, R. Pepperkok, F. Wieland, and B. Brügger (2012) Molecular recognition of a single sphingolipid species by a protein’s transmembrane domain. Nature. 481:525-529.
A.M. Ernst, F.-X. Contreras, C. Thiele, F. Wieland & B. Brügger (2012). Mutual rocognition of sphingolipid molecular species in membranes. Biochim Biophys Acta - Biomemb. 1818(11): 2616-2622.
Publications
The S-palmitoylome and DHHC-PAT interactome of Drosophila melanogaster S2R+ cells indicate a high degree of conservation to mammalian palmitoylomes. PloS one, 17(8), e0261543. https://doi.org/10.1371/journal.pone.0261543Porcellato, E., González-Sánchez, J. C., Ahlmann-Eltze, C., Elsakka, M. A., Shapira, I., Fritsch, J., Navarro, J. A., Anders, S., Russell, R. B., Wieland, F. T., & Metzendorf, C. (2022)
Proteomics Profiling of Mammalian COPII and COPI Vesicles. Cell Reports 26, 250-265. https://www.sciencedirect.com/science/article/pii/S2211124718319715Adolf, F., Rhiel, M., Hessling, B., Gao, Q., Hellwig, A., Béthune, J., and Wieland, F. (2019)
Assembly of COPI and COPII Vesicular Coat Proteins on Membranes. Annu Rev Biophys. 47:63-83. https://www.annualreviews.org/doi/abs/10.1146/annurev-biophys-070317-033259Béthune, J.*, and Wieland, F.T.* (2018)
9Å structure of COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments, Elife 6. pii: e26691.Dodonova, S.O., Aderhold, P., Kopp, J., Ganeva, I., Röhling, S., Hagen, W.J.H., Sinning, I., Wieland, F., & Briggs, J.A.G. (2017)
A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly. Science. 349:195-198.Dodonova SO, Diestelkoetter-Bachert P, von Appen A, Hagen WJH, Beck R, Beck M, Wieland F, and Briggs JAG. (2015)
Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol. Nat Commun 6, 7688. https://doi.org/10.1038/ncomms8688Hacke, M., Björkholm, A., Hellwig, P., Himmels, P., Ruiz de Almodóvar, C., Brügger, B., Wieland, F., & Ernst, A.M. (2015)
Analysis of transmembrane domains and lipid mdified peptides with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Anal. Chem. 86, 8, 3722–3726. https://doi.org/10.1021/ac500446zGerl MJ, Sachsenheimer T, Grzybek M, Coskun U, Wieland FT, & Brügger B. (2014)
Golgi phosphoprotein 3 trigers signal-mediated incorporation of glycosyltransferases into coatomer-coated (COPI) vesicles. J Biol Chem. 289(45):31319-29.Eckert ESP, Reckmann I, Hellwig A, Röhling S, Battari AE, Wieland FT, and Popoff V. (2014)
Identification of novel sphingolipid-binding motifs in mammalian membrane proteins. Biochimica et biophysica acta, 1838(8), 2066–2070. https://doi.org/10.1016/j.bbamem.2014.04.026Björkholm, P., Ernst, A. M., Hacke, M., Wieland, F., Brügger, B., & von Heijne, G. (2014)
Comparative lipidomics analysis of HIV-1 particles and their producer cell membrane in different cell lines. Cellular microbiology, 15(2), 292–304. https://doi.org/10.1111/cmi.12101Lorizate, M., Sachsenheimer, T., Glass, B., Habermann, A., Gerl, M. J., Kräusslich, H. G., & Brügger, B. (2013)
Differential transport of Influenza A neuraminidase signal anchor peptides to the plasma membrane. FEBS letters, 587(9), 1411–1417. https://doi.org/10.1016/j.febslet.2013.03.019Ernst, A. M., Zacherl, S., Herrmann, A., Hacke, M., Nickel, W., Wieland, F. T., & Brügger, B. (2013)
Mcp1 and Mcp2, two novel proteins involved in mitochondrial lipid homeostasis. J Cell Sci. 126(Pt 16):3563-74.Tan T, Ozbalci C, Brügger B, Rapaport D, Dimmer KS. (2013)
Quantitative analysis of cellular lipids by nano-electrospray ionization mass spectrometry. Methods Mol Biol. 1033:3-20. doi: 10.1007/978-1-62703-487-6_1.Ozbalci C, Sachsenheimer T, Brügger B. (2013)
Scission of COPI and COPII vesicles is independent of GTP hydrolysis. Traffic. 14(8):922-32.Adolf F, Herrmann A, Hellwig A, Beck R, Brügger B, Wieland FT. (2013)
Sphingolipids as modulators of membrane proteins. Biochim Biophys Acta. Nov 5. doi:pii: S1388-1981(13)00239-4. 10.1016/ j.bbalip.2013.10.016. [Epub ahead of print]. Review.Ernst AM, Brügger B. (2013)
Distinct role of subcomplexes of the COPI coat in the regulation of ArfGAP2 activity. Traffic, 13(6), 849–856. https://doi.org/10.1111/j.1600-0854.2012.01349.xPevzner, I., Strating, J., Lifshitz, L., Parnis, A., Glaser, F., Herrmann, A., Brügger, B., Wieland, F., & Cassel, D. (2012)
Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes. Mol Biol Cell. 23(20):3927-35.Krumpe K, Frumkin I, Herzig Y, Rimon N, Özbalci C, Brügger B, Rapaport D, Schuldiner M. (2012)
Impaired neurotransmission in ether lipid-deficient nerve terminals. Hum Mol Genet. 21(12):2713-24Brodde A, Teigler A, Brugger B, Lehmann WD, Wieland F, Berger J, Just WW. (2012)
Large membrane domains in hair bundles specify spatially constricted radixin activation. J Neurosci. 32(13):4600-9.Zhao H, Williams DE, Shin JB, Brügger B, Gillespie PG. (2012)
Molecular recognition of a single sphingolipid species by a protein's transmembrane domain. Nature, 481(7382), 525–529. https://doi.org/10.1038/nature10742Contreras, F. X., Ernst, A. M., Haberkant, P., Björkholm, P., Lindahl, E., Gönen, B., Tischer, C., Elofsson, A., von Heijne, G., Thiele, C., Pepperkok, R., Wieland, F., & Brügger, B. (2012)
Mutual recognition of sphingolipid molecular species in membranes. Biochim Biophys Acta 1818(11):2616-2622.Ernst AM, Contreras FX, Thiele C, Wieland F, Brügger B. (2012)
Poxvirus membrane biogenesis: rupture not disruption. Cell Microbiol. doi: 10.1111/cmi.12072. [Epub ahead of print]Krijnse Locker J, Chlanda P, Sachsenheimer T, Brügger B. (2012)
Sphingomyelin organization is required for vesicle biogenesis at the Golgi complex. The EMBO journal, 31(24), 4535–4546. https://doi.org/10.1038/emboj.2012.317Duran, J. M., Campelo, F., van Galen, J., Sachsenheimer, T., Sot, J., Egorov, M. V., Rentero, C., Enrich, C., Polishchuk, R. S., Goñi, F. M., Brügger, B., Wieland, F., & Malhotra, V. (2012)
The Structures of COPI-Coated Vesicles Reveal Alternate Coatomer Conformations and Interactions. Science 336(6087): 1451-4.Faini M, Prinz S, Beck R, Schorb M, Riches JD, Bacia K, Brügger B, Wieland FT, Briggs JA. (2012)
A global circadian repressor controls antiphasic expression of metabolic genes in Neurospora. Mol Cell. 2011; doi:10.1016/j.molcel.2011.10.019.Sancar, G., Sancar, C., Brügger, B., Ha, N., Sachsenheimer, T., Gin, E., Wdowik, S., Lohmann, I., Wieland, F., Höfer, T., Diernfellner, A. and Brunner, M. (2011)
Biochemical and morphological properties of hepatitis C virus particles and determination of their lipidome. J Biol Chem. 286(4):3018-32.Merz A, Long G, Hiet MS, Brügger B, Chlanda P, Andre P, Wieland F, Krijnse-Locker J, Bartenschlager R. (2011)
Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission. J Cell Biol. 194(5):765-77.Beck R, Prinz S, Diestelkötter-Bachert P, Röhling S, Adolf F, Hoehner K, Welsch S, Ronchi P, Brügger B, Briggs JA, Wieland F. (2011)
COPI Budding within the Golgi Stack. Cold Spring Harb Perspect Biol. 3(11). Review.Popoff V, Adolf F, Brügger B, Wieland F. (2011)
Encephalopathy caused by ablation of very long acyl chain ceramide synthesis may be largely due to reduced galactosylceramide levels. J Biol Chem. 286(34):30022-33.Ben-David O, Pewzner-Jung Y, Brenner O, Laviad EL, Kogot-Levin A, Weissberg I, Biton IE, Pienik R, Wang E, Kelly S, Alroy J, Raas-Rothschild A, Friedman A, Brügger B, Merrill AH Jr, Futerman AH. (2011)
GAPs in the context of COPI: Enzymes, coat components or both? Cell Logist. 1(2):52-54. Review.Beck R, Brügger B, Wieland F. (2011)
Glucocorticoid Activity during Lung Maturation Is Essential in Mesenchymal and Less in Alveolar Epithelial Cells. Mol Endocrinol. 25(8):1280-8.Habermehl D, Parkitna JR, Kaden S, Brügger B, Wieland F, Gröne HJ, Schütz G. (2011)
Human regulatory T cells rapidly suppress T Cell Receptor-induced calcium signals, NF-κB and NFAT activation in conventional CD4+CD25- T cells. Sci Signal. ra90 DOI: 10.1126/scisignal.2002179Schmidt, A, Oberle, N, Weiß, EM, Vobis, D, Frischbutter, S, Baumgrass, R, Falk, CS, Haag, M, Brügger, B, Lin, H, Mayr, GW, Reichardt, P, Gunzer, M, Suri-Payer E, Krammer PH. (2011)
Peroxisomal alterations in Alzheimer's disease. Acta Neuropathol. 122(3):271-83.Kou J, Kovacs GG, Höftberger R, Kulik W, Brodde A, Forss-Petter S, Hönigschnabl S, Gleiss A, Brügger B, Wanders R, Just W, Budka H, Jungwirth S, Fischer P, Berger J. (2011)
Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of SRC kinases. Genome Res. 21(11):1955-68.Ritzerfeld J, Remmele S, Wang T, Temmerman K, Brügger B, Wegehingel S, Tournaviti S, Strating JR, Wieland FT, Neumann B, Ellenberg J, Lawerenz C, Hesser J, Erfle H, Pepperkok R, Nickel W. (2011)
Recombinant heptameric coatomer complexes: novel tools to study isoform-specific functions. Traffic 12(6):682-92Sahlmüller MC, Strating JR, Beck R, Eckert P, Popoff V, Haag M, Hellwig A, Berger I, Brügger B, Wieland FT. (2011)
Self-segregation of myelin membrane lipids in model membranes. Biophys J. 101(11):2713-20.Yurlova L, Kahya N, Aggarwal S, Kaiser HJ, Chiantia S, Bakhti M, Pewzner-Jung Y, Ben-David O, Futerman AH, Brügger B, Simons M. (2011)
Several ADP-ribosylation factor (Arf) isoforms support COPI vesicle formation. J Biol Chem. 286(41):35634-42.Popoff V, Langer JD, Reckmann I, Hellwig A, Kahn RA, Brügger B, Wieland FT. (2011)
Specificity of intramembrane protein-lipid interactions. Cold Spring Harb Perspect Biol. 3(6). pii: a004705. Review.Contreras FX, Ernst AM, Wieland F, Brügger B. (2011)
Structural and biochemical consequences of NF1 associated nontruncating mutations in the Sec14-PH module of neurofibromin. Hum Mutat. 32(2):191-7.Welti S, Kühn S, D'Angelo I, Brügger B, Kaufmann D, Scheffzek K. (2011)
Targeting of Nbp1 to the inner nuclear membrane is essential for spindle pole body duplication. EMBO J. 30(16):3337-52.Kupke T, Di Cecco L, Müller HM, Neuner A, Adolf F, Wieland F, Nickel W, Schiebel E. (2011)
Tumor-specific silencing of COPZ2 gene encoding coatomer protein complex subunit ζ 2 renders tumor cells dependent on its paralogous gene COPZ1. Proc Natl Acad Sci U S A. 108(30):12449-54.Shtutman M, Baig M, Levina E, Hurteau G, Lim CU, Broude E, Nikiforov M, Harkins TT, Carmack CS, Ding Y, Wieland F, Buttyan R, Roninson IB. (2011)
A critical role for ceramide synthase 2 in liver homeostasis: I. alterations in lipid metabolic pathways. J Biol Chem. 285(14):10902-10.Pewzner-Jung Y, Park H, Laviad EL, Silva LC, Lahiri S, Stiban J, Erez-Roman R, Brügger B, Sachsenheimer T, Wieland F, Prieto M, Merrill AH Jr, Futerman AH. (2010)
A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4. EMBO J. 29(12):1976-87.Osman C, Haag M, Wieland FT, Brügger B, Langer T. (2010)
Determinants of specificity at the protein-lipid interface in membranes. FEBS Lett. 584(9):1713-20. Review.Ernst AM, Contreras FX, Brügger B, Wieland F. (2010)
Genetic evidence for functional interaction of the Escherichia coli signal recognition particle receptor with acidic lipids in vivo. J Biol Chem. 285(52):40508-14.Erez E, Stjepanovic G, Zelazny AM, Brügger B, Sinning I, Bibi E. (2010)
Induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranes. Proc Natl Acad Sci U S A. 107(15):6876-81.Lavieu G, Orci L, Shi L, Geiling M, Ravazzola M, Wieland F, Cosson P, Rothman JE. (2010)
Membrane deformation and separation. F1000 Biol Rep. 2. pii: 35.Beck R, Brügger B, Wieland FT. (2010)
Sphingolipid rheostat alterations related to transformation can be exploited for specific induction of lysosomal cell death in murine and human glioma. Glia 58(11):1364-83.Mora R, Dokic I, Kees T, Hüber CM, Keitel D, Geibig R, Brügger B, Zentgraf H, Brady NR, Régnier-Vigouroux A. (2010)
ArfGAP1 activity and COPI vesicle biogenesis. Traffic. 10(3):307-15.Beck R, Adolf F, Weimer C, Bruegger B, Wieland FT. (2009)
Cholesterol regulates the endoplasmic reticulum exit of the major membrane protein P0 required for peripheral myelin compaction. J Neurosci. 29(19):6094-104.Saher G, Quintes S, Möbius W, Wehr MC, Krämer-Albers EM, Brügger B, Nave KA. (2009)
Following the fate in vivo of COPI vesicles generated in vitro. Traffic. 10(8):994-1005.Rutz C, Satoh A, Ronchi P, Brügger B, Warren G, Wieland FT. (2009)
Journeys through the Golgi--taking stock in a new era. J Cell Biol. 187(4):449-53. Review.Emr S, Glick BS, Linstedt AD, Lippincott-Schwartz J, Luini A, Malhotra V, Marsh BJ, Nakano A, Pfeffer SR, Rabouille C, Rothman JE, Warren G, Wieland FT. (2009)
Probing HIV-1 membrane liquid order by Laurdan staining reveals producer cell-dependent differences. J Biol Chem. 284(33):22238-47.Lorizate M, Brügger B, Akiyama H, Glass B, Müller B, Anderluh G, Wieland FT, Kräusslich HG. (2009)
The COPI system: molecular mechanisms and function. FEBS Lett. 583(17):2701-9. Review.
Corrigendum in: FEBS Lett. 583(21):3541Beck R, Rawet M, Wieland FT, Cassel D. (2009)
The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria. J Cell Biol. 184(4):583-96.Osman C, Haag M, Potting C, Rodenfels J, Dip PV, Wieland FT, Brügger B, Westermann B, Langer T. (2009)
A conformational change in the alpha-subunit of coatomer induced by ligand binding to gamma-COP revealed by single-pair FRET. Traffic 9, 597-607. https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0854.2007.00697.xLanger, JD, Roth, CM, Béthune, J, Stoops, EH, Brügger, B, Herten, DP, and Wieland, FT* (2008)
Arf1-GTP-induced tubule formation suggests a function of Arf family proteins in curvature acquisition at sites of vesicle budding. J Biol Chem. 283(41):27717-23.Krauss M, Jia JY, Roux A, Beck R, Wieland FT, De Camilli P, Haucke V. (2008)
Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science. 319(5867):1244-7.Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F, Schwille P, Brügger B, Simons M. (2008)
Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking. J Cell Biol. 183(4):725-35.Weimer C, Beck R, Eckert P, Reckmann I, Moelleken J, Brügger B, Wieland F. (2008)
Distinct binding sites for the ATPase and substrate-binding domain of human Hsp70 on the cell surface of antigen presenting cells. Mol Immunol. 45(15):3974-83.Zitzler S, Hellwig A, Hartl FU, Wieland F, Diestelkötter-Bachert P. (2008)
Finally: The digital, democratic age of scientific abstracts. FEBS Lett. 582(8):1169.Superti-Furga G, Wieland F, Cesareni G. (2008)
Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proc Natl Acad Sci U S A 105, 11731-11736. → Highlighted by the Faculty of 1000 (F1000.com/1119165). http://www.pnas.org/content/105/33/11731Beck, R, Sun, Z, Adolf, F, Rutz, C, Bassler, J, Wild, K, Sinning, I, Hurt, E, Brügger, B, Béthune, J*, and Wieland, F* (2008)
Mutation of a single residue, beta-glutamate-20, alters protein-lipid interactions of light harvesting complex II. Mol Microbiol. 67(1):63-77.Kwa LG, Wegmann D, Brügger B, Wieland FT, Wanner G, Braun P. (2008)
Protein-sphingolipid interactions within cellular membranes. J Lipid Res. 49(1):251-62.Haberkant P, Schmitt O, Contreras FX, Thiele C, Hanada K, Sprong H, Reinhard C, Wieland FT, Brügger B. (2008)
Axonal loss and neuroinflammation caused by peroxisome-deficient oligodendrocytes. Nat Genet. 39(8):969-76.Kassmann CM, Lappe-Siefke C, Baes M, Brügger B, Mildner A, Werner HB, Natt O, Michaelis T, Prinz M, Frahm J, Nave KA. (2007)
Conformational changes of coat proteins during vesicle formation. FEBS Lett. 581: 2083-2088.Langer JD, Stoops EH, Béthune J, Wieland FT. (2007)
Differential localization of coatomer complexes isoforms within the Golgi apparatus. Proc. Natl. Acad. Sci. USA 104: 4425-4430.Moelleken J, Malsam J, Betts MJ, Movafeghi A, Reckmann I, Meissner I, Hellwig A, Russell RB, Söllner T, Brügger B, Wieland FT. (2007)
Dual roles of the transmembrane protein p23/TMP21 in the modulation of amyloid precursor protein metabolism. Mol. Neurodegener. 8, 2:4.Vetrivel KS, Gong P, Bowen JW, Cheng H, Chen Y, Carter M, Nguyen PD, Placanica L, Wieland FT, Li YM, Kounnas MZ, Thinakaran G. (2007)
Human immunodeficiency virus type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains. Retrovirology. 4:70Brügger B, Krautkrämer E, Tibroni N, Munte CE, Rauch S, Leibrecht I, Glass B, Breuer S, Geyer M, Kräusslich HG, Kalbitzer HR, Wieland FT, Fackler OT. (2007)
Involvement of a Golgi-resident GPI-anchored Protein in Maintenance of the Golgi Structure. Mol. Biol. Cell 18:1261-1271.Li X, Kaloyanova D, van Eijk M, Eerland R, van der Goot G, Oorschot V, Klumperman J, Lottspeich F, Starkuviene V, Wieland FT, Helms JB. (2007)
Multiple and stepwise interactions between coatomer and ADP-ribosylation factor-1 (Arf1)-GTP. Traffic 8: 582-593.Sun Z, Anderl F, Fröhlich K, Zhao L, Hanke S, Brügger B, Wieland F, Béthune J. (2007)
The structure of the regulatory domain of the adenylyl cyclase Rv1264 from Mycobacterium tuberculosis with bound oleic acid. J. Mol. Biol. 369: 1282-1295.Findeisen F, Linder JU, Schultz A, Schultz JE, Brügger B, Wieland F, Sinning I, Tews I. (2007)
Coatomer, the coat protein of COPI transport vesicles, discriminates endoplasmic reticulum residents from p24 proteins. Mol. Cell. Biol. 26:8011-8021.J. Béthune, M. Kol, J. Hoffmann, I. Reckmann, B. Brügger, F. Wieland (2006)
COPI-mediated transport. J. Membr. Biol. 211:65-79.J. Béthune, F. Wieland, J. Moelleken (2006)
Molecular anatomy of a trafficking organelle. Cell 127(4):831-46.Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, Rammner B, Gräter F, Hub JS, De Groot BL, Mieskes G, Moriyama Y, Klingauf J, Grubmüller H, Heuser J, Wieland F, Jahn R. (2006)
Molecular Anatomy of a Trafficking Organelle. Cell 127:831-846.S. Takamori, M. Holt, K. Stenius, E.A. Lemke, M. Grønborg, D. Riedel, H. Urlaub, S. Schenck, B. Brügger, P. Ringler, S.A. Müller, B. Rammner, F. Gräter, J.S. Hub, B.L. De Groot, G. Mieskes, Y. Moriyama, J. Klingauf, H. Grubmüller, J. Heuser, F. Wieland, and R. Jahn (2006)
The HIV lipidome: A raft with an unusual composition. Proc. Natl. Acad. Sci. USA 103:2641-2646. (10.452)B. Brügger, B. Glass, P. Haberkant, I. Leibrecht, F.T. Wieland and H.-G. Kräusslich. (2006)
High cholesterol level is essential for myelin membrane growth. Nat Neurosci. 8(4):468-75.Saher G, Brügger B, Lappe-Siefke C, Möbius W, Tozawa R, Wehr MC, Wieland F, Ishibashi S, Nave KA. (2005)
Discriminatory aptamer reveals serum response element transcription regulated by cytohesin-2. Proc. Natl. Acad. Sci. U.S.A. 101:11221-11226.M. G. Theis, A. Knorre, B. Kellersch, J. Moelleken, F. T. Wieland, W. Kolanus, and M. Famulok. (2004)
Identification and biophysical characterization of a very-long-chain-fatty-acid-substituted phosphatidylinositol in yeast subcellular membranes. Biochem. J. 381:941-949.R. Schneiter, B. Brügger, C. M. Amann, G. D. Prestwich, R. F. Epand, G. Zellnig, F. T. Wieland, and R. M. Epand. (2004)
Mechanismen der COPI-Vesikelbiogenese. Biospektrum I, 30-33 (Review).B. Brügger and F. T. Wieland (2004)
Novel isotypic g/z-subunits reveal three coatomer complexes in mammals. Mol. Cell. Biol. 3, 1070-1080. Supplemental Materials [PDF]D. Wegmann, P. Hess, C. Baier, F. T. Wieland and C. Reinhard (2004)
The membrane domains occupied by glycosylphosphatidylinositol-anchored prion protein and Thy-1 differ in lipid composition. J Biol Chem. 279(9):7530-6.Brügger B, Graham C, Leibrecht I, Mombelli E, Jen A, Wieland F, Morris R. (2004)
Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p. EMBO Rep. 4(12):1156-62.Neumann S, Petfalski E, Brügger B, Grosshans H, Wieland F, Tollervey D, Hurt E. (2003)
Functional reconstitution of COPI coat assembly and disassembly using chemically defined components. Proc. Natl. Acad Sci. U.S.A. 100:8253-8257.Constanze Reinhard, Michael Schweikert, Felix T. Wieland, and Walter Nickel (2003)
Glutamate binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts. Proc. Natl. Acad. Sci. USA 100: 10219-10224.Eroglu, C., Brügger, B., Wieland, F. & Sinning, I. (2003)
Inactivation of ether lipid biosynthesis causes male infertility, defects in eye development and optic nerve hypoplasia in mice. Hum Mol Genet. 12(15):1881-95.Rodemer C, Thai TP, Brügger B, Kaercher T, Werner H, Nave KA, Wieland F, Gorgas K, Just WW. (2003)
Intra-Golgi protein transport depends on a cholesterol balance in the lipid membrane. J Biol Chem. 278(52):53112-22.Stüven E, Porat A, Shimron F, Fass E, Kaloyanova D, Brügger B, Wieland FT, Elazar Z, Helms JB. (2003)
The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1. J Biol Chem. 278(32):29560-70.A. Kruljac-Letunic, J. Moelleken, A. Kallin, F. T. Wieland, A. Blaukat (2003)
CD40, an extracellular receptor for binding and uptake of Hsp70-peptide complexes. J Cell Biol. 158(7):1277-85.T. Becker, F. U. Hartl, F. T. Wieland (2002)
Oligomeric state and stoichiometry of p24 proteins in the early secretory pathway. J Biol Chem. 277(48):46504-11.Jenne N, Frey K, Brügger B, Wieland FT. (2002)
The Sur7p family defines novel cortical domains in Saccharomyces cerevisiae, affects sphingolipid metabolism, and is involved in sporulation. Mol Cell Biol. 22(3):927-34.Young ME, Karpova TS, Brügger B, Moschenross DM, Wang GK, Schneiter R, Wieland FT, Cooper JA. (2002)
Vesicular transport: the core machinery of COPI recruitment and budding. J Cell Sci. 115(Pt 16):3235-40. Review.Nickel W, Brügger B, Wieland FT. (2002)
Membrane Trafficking: How Transport Vesicles Fuse With Target Membranes in "Protein Modules in Cellular Signalling", eds Heilmeyer L. and Friedrich P. (IOS Press Amsterdam), pp. 257-261 (Review).Walter Nickel, Britta Brügger and Felix T. Wieland (2001)
Molecular Mechanisms of COPI Vesicle Formation in "Protein Modules in Cellular Signalling", eds. Heilmeyer L. and Friedrich P. (IOS Press Amsterdam), pp. 262-267 (Review).Britta Brügger, Walter Nickel, and Felix T. Wieland (2001)
Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23. The EMBO journal, 20(23), 6751–6760. https://doi.org/10.1093/emboj/20.23.6751Gommel, D. U., Memon, A. R., Heiss, A., Lottspeich, F., Pfannstiel, J., Lechner, J., Reinhard, C., Helms, J. B., Nickel, W., & Wieland, F. T. (2001)
Sphingomyelin-enriched microdomains at the Golgi complex. Mol Biol Cell 12(6):1819-33.Gkantiragas I, Brügger B, Stüven E, Kaloyanova D, Li XY, Löhr K, Lottspeich F, Wieland FT, Helms JB. (2001)
Evidence for segregation of sphingomyelin and cholesterol during formation of COPI-coated vesicles. J Cell Biol. 151(3):507-18.Brügger B, Sandhoff R, Wegehingel S, Gorgas K, Malsam J, Helms JB, Lehmann WD, Nickel W, Wieland FT. (2000)
Receptor-Dependent Formation of COPI-Coated Vesicles From Chemically Defined Donor Liposomes. Methods Enzymol. 329:388-404.Walter Nickel and Felix T. Wieland (2000)
A role for ADP-ribosylation factor in the control of cargo uptake during COPI-coated vesicle biogenesis. FEBS Lett. 462:267-272.Jörg Malsam, Daniel Gommel, Felix. T. Wieland, and Walter Nickel (1999)
Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors. Cell 96, 495-506.M. Bremser, W. Nickel, M. Schweikert, M. Ravazzola, M. Amherd, C.A. Hughes, T.H. Söllner, J. E. Rothman, and F. T. Wieland (1999)
Determination of cholesterol at the low picomole level by nano-electrospray ionization tandem mass spectrometry. J Lipid Res. 40(1):126-32.Sandhoff R, Brügger B, Jeckel D, Lehmann WD, Wieland FT. (1999)
Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane. J Cell Biol. 146(4):741-54.Schneiter R, Brügger B, Sandhoff R, Zellnig G, Leber A, Lampl M, Athenstaedt K, Hrastnik C, Eder S, Daum G, Paltauf F, Wieland FT, Kohlwein SD. (1999)
GTP-dependent Binding of ARF to Coatomer in Close Proximity to the Binding Site for Dilysine Retrieval Motifs and p23. J. Biol. Chem. 274, 14198-14203.L. Zhao, B. Helms, J. Brunner, and F. Wieland (1999)
Maturation of the axonal plasma membrane requires upregulation of sphingomyelin synthesis and formation of protein-lipid complexes. EMBO J. 18(7):1761-71.Ledesma MD, Brügger B, Bünning C, Wieland FT, Dotti CG. (1999)
Mechanisms of vesicle formation: insights from the COP system. Curr. Opin. Cell Biol. 11(4), 440-446.Wieland, F., & Harter, C. (1999)
p24 and p23, the major transmem-brane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway. FEBS Lett. 447:179-185.D. Gommel, L. Orci, E.M. Emig, M.J. Hannah, M. Ravazzola, W. Nickel, J.B. Helms, F.T. Wieland and K. Sohn (1999)
Receptor induced polymerisation of coatomer. Proc. Natl. Acad. Sci U.S.A. 96, 1224-1228.C. Reinhard, C. Harter, M. Bremser, B. Brügger, K. Sohn. B. Helms, and F. Wieland (1999)
A putative heterotrimeric G protein inhibits the fusion of COPI-coated vesicles. Segregation of heterotrimeric G proteins from COPI-coated vesicles. J Biol Chem. 273(24):15203-8.Helms JB, Helms-Brons D, Brügger B, Gkantiragas I, Eberle H, Nickel W, Nürnberg B, Gerdes HH, Wieland FT. (1998)
A single binding site for di-lysine motifs and p23 within the g-subunit of coatomer. Proc. Natl. Acad. Sci. USA. 95,11649-11654C. Harter, and F. Wieland (1998)
Biosynthetic Protein Transport Through the Early Secretory Pathway. Histochem. Cell Biol. 109:477-486 (Review).Walter Nickel and Felix T. Wieland (1998)
Functional organization of the Golgi apparatus in glycosphingolipid biosynthesis. Lactosylceramide and subsequent glycosphingolipids are formed in the lumen of the late Golgi. J.Biol.Chem. 273:2939-2946.Lannert, H., K. Gorgas, I. Meissner, F.T. Wieland, and D. Jeckel (1998)
Intracellular Transport of Glycoproteins and Glycolipids. Trends Glycosci. Glycotechnolog. 10:1-11 (Review).Walter Nickel, Britta Brügger, and Felix T. Wieland (1998)
Peroxisome biogenesis: involvement of ARF and coatomer. J. Cell Biol. 141, 373-383.M. Passreiter, M. Anton, D. Lay, R. Frank, C. Harter, F.T. Wieland, K. Gorgas and W.W. Just (1998)
Protein and lipid sorting between the endoplasmic reticulum and the Golgi complex. Semin Cell Dev Biol. 9(5):493-501. Review.Nickel W, Brügger B, Wieland FT. (1998)
Reversible dissociation of coatomer: Functional characterization of a b/d-coat protein subcomplex. Proc. Natl. Acad. Sci. U.S.A., 95, 2140-2145.J. Pavel, C. Harter, and F. Wieland (1998)
Uptake by COPI-coated vesicles of both anterograde and retrograde cargo is inhibited by GTPgammaS in vitro. J. Cell Sci. 111:3081-3090.Walter Nickel, Jörg Malsam, Karin Gorgas, Mariella Ravazzola, Nicole Jenne, J. Bernd Helms, and Felix T. Wieland (1998)
Biogenesis of COPI-Coated Transport Vesicles. FEBS Lett. 413:395-400 (Review).Walter Nickel and Felix T. Wieland (1997)
Direct and GTP-dependent interaction of ADP ribosylation factor 1 with coatomer subunit beta. Proc Natl Acad Sci USA 94(9):4418-23.Zhao L, Helms JB, Brügger B, Harter C, Martoglio B, Graf R, Brunner J, Wieland FT. (1997)
p23, a major COPI-vesicle membrane protein, constitutively cycles through the early secretory pathway. Proc. Natl. Acad. Sci. U.S.A. 94, 11393-11398.W. Nickel, K. Sohn, C. Bünning, and F. Wieland (1997)
Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. Proc Natl Acad Sci USA 94(6):2339-44.Brügger B, Erben G, Sandhoff R, Wieland FT, Lehmann WD. (1997)
A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding. J. Cell Biol. 135, 1238-1248K. Sohn, L. Orci, M. Ravazzola, M. Amherdt, M. Bremser, F. Lottspeich, K. Fiedler, J. B. Helms, and F. Wieland (1996)
Architecture of coatomer: molecular characterization of d-COP and protein interactions within the complex. J. Cell Biol. 135, 53-61.D. Faulstich, S. Auerbach, L. Orci, M. Ravazzola, S. Wegehingel, F. Lottspeich, G. Stenbeck, C. Harter, F. T. Wieland, and H. Tschochner (1996)
Forskolin stimulates detoxification of brefeldin A. J. Biol. Chem. 271:15870-15873.Walter Nickel, J. Bernd Helms, Richard E. Kneusel, and Felix T. Wieland (1996)
Non-clathrin-coat protein g, a subunit of coatomer, binds to the cytoplasmic dilysine motif of membrane proteins of the early secretory pathway. Proc. Natl. Acad. Sci. U.S.A. 93, 1902-1906C. Harter, J. Pavel, F. Coccia, E. Draken, S. Wegehingel, H. Tschochner, and F. Wieland (1996)
Protein sorting by transport vesicles. Science 272, 227-234.*J. E. Rothman and F. T. Wieland (1996)
Non-clathrin-coat protein a is a conserved subunit of coatomer and in Saccharomyces cerevisiae is essential for growth. Proc. Natl. Acad. Sci. U.S.A. 92, 3229-3233.B. Gerich, L. Orci, H. Tschochner, F. Lottspeich, M. Ravazzola, M. Amherdt, F. Wieland, and C. Harter (1995)
VIP21/caveolin is a cholesterol-binding protein. Proc. Natl. Acad. Sci. U.S.A. 92, 10339-10343M. Murata, J. Peränen, R. Schreiner, F. Wieland, T. V. Kurzchalia, and K. Simons (1995)
Analysis of bacterial glycoproteins. Methods Mol Biol 14:119-29.Lechner J, Wieland F. (1993)
b'-COP, a Novel Subunit of Coatomer. EMBO J. 12, 2841-2845G. Stenbeck, C. Harter, A. Brecht, D. Herrmann, F. Lottspeich, L. Orci, and F. T. Wieland (1993)
Membrane topology of the 22 kDa integral peroxisomal membrane protein. FEBS Lett. 315(3), 217-22K.Kaldi,P. Diestelkotter,G. Stenbeck,S. Auerbach,U. Jakle,H.J. Magert,F.T. Wieland and W.W. Just (1993)
Glucosylceramide is synthesized at the cytosolic surface of various Golgi subfractions. J. Cell Biol. 117, 259-267D. Jeckel, A. Karrenbauer, K. N. J. Burger, G. van Meer, and F. Wieland (1992)
A Coat Subunit of Golgi-Derived non-Clathrin Coated Vesicles with Homology to the Clathrin Coated Vesicle Coat Protein b-Adaptin. Nature 349, 215-220.T. Serafini, G. Stenbeck, A. Brecht, F. Lottspeich, L. Orci, J. E. Rothman, and F. Wieland (1991)
The Rate of Bulk Flow from the Golgi to the Plasma Membrane. Cell 63, 259-267.A. Karrenbauer, D. Jeckel. W. Just, R. Birk, R. R. Schmidt, J. E. Rothman, and F. Wieland (1990)
Purification of an N-Ethylmaleimide-Sensitive Protein Catalyzing Vesicular Transport. Proc. Natl. Acad. Sci.U.S.A. 85, 7852-7856.M. R. Block, B. S. Glick, C. A. Wilcox, F. Wieland, and J. Rothman (1988)
The Rate of Bulk Flow from the Endoplasmic Reticulum to the Cell Surface. Cell 50, 289-300.F. Wieland, M. Gleason, T. Serafini, J. Rothman (1987)
Asparaginyl-N-Acetylgalactosamine: Linkage Unit of Halobacterial Glycosaminoglycan. J. Biol. Chem. 261, 1020-1024.G. Paul, F. Lottspeich, and F. Wieland (1986)
Iduronic acid is a constituent of sulfated dolichyl phosphate oligosaccharides in halobacteria. FEBS Lett. 195:77-81.Wieland F, Lechner J, Sumper M. (1986)
Sulfated Dolicholphosphate Oligosaccharides are Transiently Methylated during Biosynthesis of Halobacterial Glycoproteins. System. Appl. Microbiol. 7:286-392.Wieland F, Lechner J, Sumper M. (1986)
Biosynthesis of sulfated saccharides N-glycosidically linked to the protein via glucose. Purification and identification of sulfated dolichyl monophosphoryl tetrasaccharides from halobacteria. J Biol Chem 260:860-6.Lechner J, Wieland F, Sumper M. (1985)
Transient methylation of dolichyl oligosaccharides is an obligatory step in halobacterial sulfated glycoprotein biosynthesis. J Biol Chem 260:8984-9.Lechner J, Wieland F, Sumper M. (1985)
Asparaginyl-Glucose: Novel Type of Carbohydrate Linkage. Proc. Natl. Acad. Sci. U.S.A 80, 5470-5474F. Wieland, R. Heitzer, and W. Schäfer (1983)
The Cell Wall Glycoprotein of Halobacteria: Structural, Functional and Biosynthetiv Aspects. Zentralbl. Bacteriol. Mikrobiol. Hyg. 1 Abt. Orig. C 3,:161-170.Wieland F, Lechner J, Sumper M. (1982)
Sulfation of a repetitive saccharide in halobacterial cell wall glycoprotein. FEBS Lett 132:319-323.Wieland F, Lechner J, Bernhardt G, Sumper M. (1981)
Distribution of Yeast Fatty Acid Synthetase subunits: Threedimensional Model of the Enzyme. Proc. Natl. Acad. Sci. U.S.A. 75, 5792-5796.F. Wieland, E. A. Siess, L. Renner, C. Verfürth, and F. Lynen (1978)
Proteomics Profiling of Mammalian COPII and COPI Vesicles. Cell Reports 26, 250-265. https://www.sciencedirect.com/science/article/pii/S2211124718319715Adolf, F., Rhiel, M., Hessling, B., Gao, Q., Hellwig, A., Béthune, J., and Wieland, F. (2019)
Assembly of COPI and COPII Vesicular Coat Proteins on Membranes. Annu Rev Biophys. 47:63-83. https://www.annualreviews.org/doi/abs/10.1146/annurev-biophys-070317-033259Béthune, J.*, and Wieland, F.T.* (2018)
9Å structure of COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments, Elife 6. pii: e26691.Dodonova, S.O., Aderhold, P., Kopp, J., Ganeva, I., Röhling, S., Hagen, W.J.H., Sinning, I., Wieland, F., & Briggs, J.A.G. (2017)
A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly. Science. 349:195-198.Dodonova SO, Diestelkoetter-Bachert P, von Appen A, Hagen WJH, Beck R, Beck M, Wieland F, and Briggs JAG. (2015)
Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol. Nat Commun 6, 7688. https://doi.org/10.1038/ncomms8688Hacke, M., Björkholm, A., Hellwig, P., Himmels, P., Ruiz de Almodóvar, C., Brügger, B., Wieland, F., & Ernst, A.M. (2015)
Analysis of transmembrane domains and lipid mdified peptides with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Anal. Chem. 86, 8, 3722–3726. https://doi.org/10.1021/ac500446zGerl MJ, Sachsenheimer T, Grzybek M, Coskun U, Wieland FT, & Brügger B. (2014)
Golgi phosphoprotein 3 trigers signal-mediated incorporation of glycosyltransferases into coatomer-coated (COPI) vesicles. J Biol Chem. 289(45):31319-29.Eckert ESP, Reckmann I, Hellwig A, Röhling S, Battari AE, Wieland FT, and Popoff V. (2014)
Identification of novel sphingolipid-binding motifs in mammalian membrane proteins. Biochimica et biophysica acta, 1838(8), 2066–2070. https://doi.org/10.1016/j.bbamem.2014.04.026Björkholm, P., Ernst, A. M., Hacke, M., Wieland, F., Brügger, B., & von Heijne, G. (2014)
Comparative lipidomics analysis of HIV-1 particles and their producer cell membrane in different cell lines. Cellular microbiology, 15(2), 292–304. https://doi.org/10.1111/cmi.12101Lorizate, M., Sachsenheimer, T., Glass, B., Habermann, A., Gerl, M. J., Kräusslich, H. G., & Brügger, B. (2013)
Differential transport of Influenza A neuraminidase signal anchor peptides to the plasma membrane. FEBS letters, 587(9), 1411–1417. https://doi.org/10.1016/j.febslet.2013.03.019Ernst, A. M., Zacherl, S., Herrmann, A., Hacke, M., Nickel, W., Wieland, F. T., & Brügger, B. (2013)
Mcp1 and Mcp2, two novel proteins involved in mitochondrial lipid homeostasis. J Cell Sci. 126(Pt 16):3563-74.Tan T, Ozbalci C, Brügger B, Rapaport D, Dimmer KS. (2013)
Quantitative analysis of cellular lipids by nano-electrospray ionization mass spectrometry. Methods Mol Biol. 1033:3-20. doi: 10.1007/978-1-62703-487-6_1.Ozbalci C, Sachsenheimer T, Brügger B. (2013)
Scission of COPI and COPII vesicles is independent of GTP hydrolysis. Traffic. 14(8):922-32.Adolf F, Herrmann A, Hellwig A, Beck R, Brügger B, Wieland FT. (2013)
Sphingolipids as modulators of membrane proteins. Biochim Biophys Acta. Nov 5. doi:pii: S1388-1981(13)00239-4. 10.1016/ j.bbalip.2013.10.016. [Epub ahead of print]. Review.Ernst AM, Brügger B. (2013)
Distinct role of subcomplexes of the COPI coat in the regulation of ArfGAP2 activity. Traffic, 13(6), 849–856. https://doi.org/10.1111/j.1600-0854.2012.01349.xPevzner, I., Strating, J., Lifshitz, L., Parnis, A., Glaser, F., Herrmann, A., Brügger, B., Wieland, F., & Cassel, D. (2012)
Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes. Mol Biol Cell. 23(20):3927-35.Krumpe K, Frumkin I, Herzig Y, Rimon N, Özbalci C, Brügger B, Rapaport D, Schuldiner M. (2012)
Impaired neurotransmission in ether lipid-deficient nerve terminals. Hum Mol Genet. 21(12):2713-24Brodde A, Teigler A, Brugger B, Lehmann WD, Wieland F, Berger J, Just WW. (2012)
Large membrane domains in hair bundles specify spatially constricted radixin activation. J Neurosci. 32(13):4600-9.Zhao H, Williams DE, Shin JB, Brügger B, Gillespie PG. (2012)
Molecular recognition of a single sphingolipid species by a protein's transmembrane domain. Nature, 481(7382), 525–529. https://doi.org/10.1038/nature10742Contreras, F. X., Ernst, A. M., Haberkant, P., Björkholm, P., Lindahl, E., Gönen, B., Tischer, C., Elofsson, A., von Heijne, G., Thiele, C., Pepperkok, R., Wieland, F., & Brügger, B. (2012)
Mutual recognition of sphingolipid molecular species in membranes. Biochim Biophys Acta 1818(11):2616-2622.Ernst AM, Contreras FX, Thiele C, Wieland F, Brügger B. (2012)
Poxvirus membrane biogenesis: rupture not disruption. Cell Microbiol. doi: 10.1111/cmi.12072. [Epub ahead of print]Krijnse Locker J, Chlanda P, Sachsenheimer T, Brügger B. (2012)
Sphingomyelin organization is required for vesicle biogenesis at the Golgi complex. The EMBO journal, 31(24), 4535–4546. https://doi.org/10.1038/emboj.2012.317Duran, J. M., Campelo, F., van Galen, J., Sachsenheimer, T., Sot, J., Egorov, M. V., Rentero, C., Enrich, C., Polishchuk, R. S., Goñi, F. M., Brügger, B., Wieland, F., & Malhotra, V. (2012)
The Structures of COPI-Coated Vesicles Reveal Alternate Coatomer Conformations and Interactions. Science 336(6087): 1451-4.Faini M, Prinz S, Beck R, Schorb M, Riches JD, Bacia K, Brügger B, Wieland FT, Briggs JA. (2012)
A global circadian repressor controls antiphasic expression of metabolic genes in Neurospora. Mol Cell. 2011; doi:10.1016/j.molcel.2011.10.019.Sancar, G., Sancar, C., Brügger, B., Ha, N., Sachsenheimer, T., Gin, E., Wdowik, S., Lohmann, I., Wieland, F., Höfer, T., Diernfellner, A. and Brunner, M. (2011)
Biochemical and morphological properties of hepatitis C virus particles and determination of their lipidome. J Biol Chem. 286(4):3018-32.Merz A, Long G, Hiet MS, Brügger B, Chlanda P, Andre P, Wieland F, Krijnse-Locker J, Bartenschlager R. (2011)
Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission. J Cell Biol. 194(5):765-77.Beck R, Prinz S, Diestelkötter-Bachert P, Röhling S, Adolf F, Hoehner K, Welsch S, Ronchi P, Brügger B, Briggs JA, Wieland F. (2011)
COPI Budding within the Golgi Stack. Cold Spring Harb Perspect Biol. 3(11). Review.Popoff V, Adolf F, Brügger B, Wieland F. (2011)
Encephalopathy caused by ablation of very long acyl chain ceramide synthesis may be largely due to reduced galactosylceramide levels. J Biol Chem. 286(34):30022-33.Ben-David O, Pewzner-Jung Y, Brenner O, Laviad EL, Kogot-Levin A, Weissberg I, Biton IE, Pienik R, Wang E, Kelly S, Alroy J, Raas-Rothschild A, Friedman A, Brügger B, Merrill AH Jr, Futerman AH. (2011)
GAPs in the context of COPI: Enzymes, coat components or both? Cell Logist. 1(2):52-54. Review.Beck R, Brügger B, Wieland F. (2011)
Glucocorticoid Activity during Lung Maturation Is Essential in Mesenchymal and Less in Alveolar Epithelial Cells. Mol Endocrinol. 25(8):1280-8.Habermehl D, Parkitna JR, Kaden S, Brügger B, Wieland F, Gröne HJ, Schütz G. (2011)
Human regulatory T cells rapidly suppress T Cell Receptor-induced calcium signals, NF-κB and NFAT activation in conventional CD4+CD25- T cells. Sci Signal. ra90 DOI: 10.1126/scisignal.2002179Schmidt, A, Oberle, N, Weiß, EM, Vobis, D, Frischbutter, S, Baumgrass, R, Falk, CS, Haag, M, Brügger, B, Lin, H, Mayr, GW, Reichardt, P, Gunzer, M, Suri-Payer E, Krammer PH. (2011)
Peroxisomal alterations in Alzheimer's disease. Acta Neuropathol. 122(3):271-83.Kou J, Kovacs GG, Höftberger R, Kulik W, Brodde A, Forss-Petter S, Hönigschnabl S, Gleiss A, Brügger B, Wanders R, Just W, Budka H, Jungwirth S, Fischer P, Berger J. (2011)
Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of SRC kinases. Genome Res. 21(11):1955-68.Ritzerfeld J, Remmele S, Wang T, Temmerman K, Brügger B, Wegehingel S, Tournaviti S, Strating JR, Wieland FT, Neumann B, Ellenberg J, Lawerenz C, Hesser J, Erfle H, Pepperkok R, Nickel W. (2011)
Recombinant heptameric coatomer complexes: novel tools to study isoform-specific functions. Traffic 12(6):682-92Sahlmüller MC, Strating JR, Beck R, Eckert P, Popoff V, Haag M, Hellwig A, Berger I, Brügger B, Wieland FT. (2011)
Self-segregation of myelin membrane lipids in model membranes. Biophys J. 101(11):2713-20.Yurlova L, Kahya N, Aggarwal S, Kaiser HJ, Chiantia S, Bakhti M, Pewzner-Jung Y, Ben-David O, Futerman AH, Brügger B, Simons M. (2011)
Several ADP-ribosylation factor (Arf) isoforms support COPI vesicle formation. J Biol Chem. 286(41):35634-42.Popoff V, Langer JD, Reckmann I, Hellwig A, Kahn RA, Brügger B, Wieland FT. (2011)
Specificity of intramembrane protein-lipid interactions. Cold Spring Harb Perspect Biol. 3(6). pii: a004705. Review.Contreras FX, Ernst AM, Wieland F, Brügger B. (2011)
Structural and biochemical consequences of NF1 associated nontruncating mutations in the Sec14-PH module of neurofibromin. Hum Mutat. 32(2):191-7.Welti S, Kühn S, D'Angelo I, Brügger B, Kaufmann D, Scheffzek K. (2011)
Targeting of Nbp1 to the inner nuclear membrane is essential for spindle pole body duplication. EMBO J. 30(16):3337-52.Kupke T, Di Cecco L, Müller HM, Neuner A, Adolf F, Wieland F, Nickel W, Schiebel E. (2011)
Tumor-specific silencing of COPZ2 gene encoding coatomer protein complex subunit ζ 2 renders tumor cells dependent on its paralogous gene COPZ1. Proc Natl Acad Sci U S A. 108(30):12449-54.Shtutman M, Baig M, Levina E, Hurteau G, Lim CU, Broude E, Nikiforov M, Harkins TT, Carmack CS, Ding Y, Wieland F, Buttyan R, Roninson IB. (2011)
A critical role for ceramide synthase 2 in liver homeostasis: I. alterations in lipid metabolic pathways. J Biol Chem. 285(14):10902-10.Pewzner-Jung Y, Park H, Laviad EL, Silva LC, Lahiri S, Stiban J, Erez-Roman R, Brügger B, Sachsenheimer T, Wieland F, Prieto M, Merrill AH Jr, Futerman AH. (2010)
A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4. EMBO J. 29(12):1976-87.Osman C, Haag M, Wieland FT, Brügger B, Langer T. (2010)
Determinants of specificity at the protein-lipid interface in membranes. FEBS Lett. 584(9):1713-20. Review.Ernst AM, Contreras FX, Brügger B, Wieland F. (2010)
Genetic evidence for functional interaction of the Escherichia coli signal recognition particle receptor with acidic lipids in vivo. J Biol Chem. 285(52):40508-14.Erez E, Stjepanovic G, Zelazny AM, Brügger B, Sinning I, Bibi E. (2010)
Induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranes. Proc Natl Acad Sci U S A. 107(15):6876-81.Lavieu G, Orci L, Shi L, Geiling M, Ravazzola M, Wieland F, Cosson P, Rothman JE. (2010)
Membrane deformation and separation. F1000 Biol Rep. 2. pii: 35.Beck R, Brügger B, Wieland FT. (2010)
Sphingolipid rheostat alterations related to transformation can be exploited for specific induction of lysosomal cell death in murine and human glioma. Glia 58(11):1364-83.Mora R, Dokic I, Kees T, Hüber CM, Keitel D, Geibig R, Brügger B, Zentgraf H, Brady NR, Régnier-Vigouroux A. (2010)
ArfGAP1 activity and COPI vesicle biogenesis. Traffic. 10(3):307-15.Beck R, Adolf F, Weimer C, Bruegger B, Wieland FT. (2009)
Cholesterol regulates the endoplasmic reticulum exit of the major membrane protein P0 required for peripheral myelin compaction. J Neurosci. 29(19):6094-104.Saher G, Quintes S, Möbius W, Wehr MC, Krämer-Albers EM, Brügger B, Nave KA. (2009)
Following the fate in vivo of COPI vesicles generated in vitro. Traffic. 10(8):994-1005.Rutz C, Satoh A, Ronchi P, Brügger B, Warren G, Wieland FT. (2009)
Journeys through the Golgi--taking stock in a new era. J Cell Biol. 187(4):449-53. Review.Emr S, Glick BS, Linstedt AD, Lippincott-Schwartz J, Luini A, Malhotra V, Marsh BJ, Nakano A, Pfeffer SR, Rabouille C, Rothman JE, Warren G, Wieland FT. (2009)
Probing HIV-1 membrane liquid order by Laurdan staining reveals producer cell-dependent differences. J Biol Chem. 284(33):22238-47.Lorizate M, Brügger B, Akiyama H, Glass B, Müller B, Anderluh G, Wieland FT, Kräusslich HG. (2009)
The COPI system: molecular mechanisms and function. FEBS Lett. 583(17):2701-9. Review.
Corrigendum in: FEBS Lett. 583(21):3541Beck R, Rawet M, Wieland FT, Cassel D. (2009)
The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria. J Cell Biol. 184(4):583-96.Osman C, Haag M, Potting C, Rodenfels J, Dip PV, Wieland FT, Brügger B, Westermann B, Langer T. (2009)
A conformational change in the alpha-subunit of coatomer induced by ligand binding to gamma-COP revealed by single-pair FRET. Traffic 9, 597-607. https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0854.2007.00697.xLanger, JD, Roth, CM, Béthune, J, Stoops, EH, Brügger, B, Herten, DP, and Wieland, FT* (2008)
Arf1-GTP-induced tubule formation suggests a function of Arf family proteins in curvature acquisition at sites of vesicle budding. J Biol Chem. 283(41):27717-23.Krauss M, Jia JY, Roux A, Beck R, Wieland FT, De Camilli P, Haucke V. (2008)
Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science. 319(5867):1244-7.Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F, Schwille P, Brügger B, Simons M. (2008)
Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking. J Cell Biol. 183(4):725-35.Weimer C, Beck R, Eckert P, Reckmann I, Moelleken J, Brügger B, Wieland F. (2008)
Distinct binding sites for the ATPase and substrate-binding domain of human Hsp70 on the cell surface of antigen presenting cells. Mol Immunol. 45(15):3974-83.Zitzler S, Hellwig A, Hartl FU, Wieland F, Diestelkötter-Bachert P. (2008)
Finally: The digital, democratic age of scientific abstracts. FEBS Lett. 582(8):1169.Superti-Furga G, Wieland F, Cesareni G. (2008)
Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proc Natl Acad Sci U S A 105, 11731-11736. → Highlighted by the Faculty of 1000 (F1000.com/1119165). http://www.pnas.org/content/105/33/11731Beck, R, Sun, Z, Adolf, F, Rutz, C, Bassler, J, Wild, K, Sinning, I, Hurt, E, Brügger, B, Béthune, J*, and Wieland, F* (2008)
Mutation of a single residue, beta-glutamate-20, alters protein-lipid interactions of light harvesting complex II. Mol Microbiol. 67(1):63-77.Kwa LG, Wegmann D, Brügger B, Wieland FT, Wanner G, Braun P. (2008)
Protein-sphingolipid interactions within cellular membranes. J Lipid Res. 49(1):251-62.Haberkant P, Schmitt O, Contreras FX, Thiele C, Hanada K, Sprong H, Reinhard C, Wieland FT, Brügger B. (2008)
Axonal loss and neuroinflammation caused by peroxisome-deficient oligodendrocytes. Nat Genet. 39(8):969-76.Kassmann CM, Lappe-Siefke C, Baes M, Brügger B, Mildner A, Werner HB, Natt O, Michaelis T, Prinz M, Frahm J, Nave KA. (2007)
Conformational changes of coat proteins during vesicle formation. FEBS Lett. 581: 2083-2088.Langer JD, Stoops EH, Béthune J, Wieland FT. (2007)
Differential localization of coatomer complexes isoforms within the Golgi apparatus. Proc. Natl. Acad. Sci. USA 104: 4425-4430.Moelleken J, Malsam J, Betts MJ, Movafeghi A, Reckmann I, Meissner I, Hellwig A, Russell RB, Söllner T, Brügger B, Wieland FT. (2007)
Dual roles of the transmembrane protein p23/TMP21 in the modulation of amyloid precursor protein metabolism. Mol. Neurodegener. 8, 2:4.Vetrivel KS, Gong P, Bowen JW, Cheng H, Chen Y, Carter M, Nguyen PD, Placanica L, Wieland FT, Li YM, Kounnas MZ, Thinakaran G. (2007)
Human immunodeficiency virus type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains. Retrovirology. 4:70Brügger B, Krautkrämer E, Tibroni N, Munte CE, Rauch S, Leibrecht I, Glass B, Breuer S, Geyer M, Kräusslich HG, Kalbitzer HR, Wieland FT, Fackler OT. (2007)
Involvement of a Golgi-resident GPI-anchored Protein in Maintenance of the Golgi Structure. Mol. Biol. Cell 18:1261-1271.Li X, Kaloyanova D, van Eijk M, Eerland R, van der Goot G, Oorschot V, Klumperman J, Lottspeich F, Starkuviene V, Wieland FT, Helms JB. (2007)
Multiple and stepwise interactions between coatomer and ADP-ribosylation factor-1 (Arf1)-GTP. Traffic 8: 582-593.Sun Z, Anderl F, Fröhlich K, Zhao L, Hanke S, Brügger B, Wieland F, Béthune J. (2007)
The structure of the regulatory domain of the adenylyl cyclase Rv1264 from Mycobacterium tuberculosis with bound oleic acid. J. Mol. Biol. 369: 1282-1295.Findeisen F, Linder JU, Schultz A, Schultz JE, Brügger B, Wieland F, Sinning I, Tews I. (2007)
Coatomer, the coat protein of COPI transport vesicles, discriminates endoplasmic reticulum residents from p24 proteins. Mol. Cell. Biol. 26:8011-8021.J. Béthune, M. Kol, J. Hoffmann, I. Reckmann, B. Brügger, F. Wieland (2006)
COPI-mediated transport. J. Membr. Biol. 211:65-79.J. Béthune, F. Wieland, J. Moelleken (2006)
Molecular anatomy of a trafficking organelle. Cell 127(4):831-46.Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, Rammner B, Gräter F, Hub JS, De Groot BL, Mieskes G, Moriyama Y, Klingauf J, Grubmüller H, Heuser J, Wieland F, Jahn R. (2006)
Molecular Anatomy of a Trafficking Organelle. Cell 127:831-846.S. Takamori, M. Holt, K. Stenius, E.A. Lemke, M. Grønborg, D. Riedel, H. Urlaub, S. Schenck, B. Brügger, P. Ringler, S.A. Müller, B. Rammner, F. Gräter, J.S. Hub, B.L. De Groot, G. Mieskes, Y. Moriyama, J. Klingauf, H. Grubmüller, J. Heuser, F. Wieland, and R. Jahn (2006)
The HIV lipidome: A raft with an unusual composition. Proc. Natl. Acad. Sci. USA 103:2641-2646. (10.452)B. Brügger, B. Glass, P. Haberkant, I. Leibrecht, F.T. Wieland and H.-G. Kräusslich. (2006)
High cholesterol level is essential for myelin membrane growth. Nat Neurosci. 8(4):468-75.Saher G, Brügger B, Lappe-Siefke C, Möbius W, Tozawa R, Wehr MC, Wieland F, Ishibashi S, Nave KA. (2005)
Discriminatory aptamer reveals serum response element transcription regulated by cytohesin-2. Proc. Natl. Acad. Sci. U.S.A. 101:11221-11226.M. G. Theis, A. Knorre, B. Kellersch, J. Moelleken, F. T. Wieland, W. Kolanus, and M. Famulok. (2004)
Identification and biophysical characterization of a very-long-chain-fatty-acid-substituted phosphatidylinositol in yeast subcellular membranes. Biochem. J. 381:941-949.R. Schneiter, B. Brügger, C. M. Amann, G. D. Prestwich, R. F. Epand, G. Zellnig, F. T. Wieland, and R. M. Epand. (2004)
Mechanismen der COPI-Vesikelbiogenese. Biospektrum I, 30-33 (Review).B. Brügger and F. T. Wieland (2004)
Novel isotypic g/z-subunits reveal three coatomer complexes in mammals. Mol. Cell. Biol. 3, 1070-1080. Supplemental Materials [PDF]D. Wegmann, P. Hess, C. Baier, F. T. Wieland and C. Reinhard (2004)
The membrane domains occupied by glycosylphosphatidylinositol-anchored prion protein and Thy-1 differ in lipid composition. J Biol Chem. 279(9):7530-6.Brügger B, Graham C, Leibrecht I, Mombelli E, Jen A, Wieland F, Morris R. (2004)
Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p. EMBO Rep. 4(12):1156-62.Neumann S, Petfalski E, Brügger B, Grosshans H, Wieland F, Tollervey D, Hurt E. (2003)
Functional reconstitution of COPI coat assembly and disassembly using chemically defined components. Proc. Natl. Acad Sci. U.S.A. 100:8253-8257.Constanze Reinhard, Michael Schweikert, Felix T. Wieland, and Walter Nickel (2003)
Glutamate binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts. Proc. Natl. Acad. Sci. USA 100: 10219-10224.Eroglu, C., Brügger, B., Wieland, F. & Sinning, I. (2003)
Inactivation of ether lipid biosynthesis causes male infertility, defects in eye development and optic nerve hypoplasia in mice. Hum Mol Genet. 12(15):1881-95.Rodemer C, Thai TP, Brügger B, Kaercher T, Werner H, Nave KA, Wieland F, Gorgas K, Just WW. (2003)
Intra-Golgi protein transport depends on a cholesterol balance in the lipid membrane. J Biol Chem. 278(52):53112-22.Stüven E, Porat A, Shimron F, Fass E, Kaloyanova D, Brügger B, Wieland FT, Elazar Z, Helms JB. (2003)
The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1. J Biol Chem. 278(32):29560-70.A. Kruljac-Letunic, J. Moelleken, A. Kallin, F. T. Wieland, A. Blaukat (2003)
CD40, an extracellular receptor for binding and uptake of Hsp70-peptide complexes. J Cell Biol. 158(7):1277-85.T. Becker, F. U. Hartl, F. T. Wieland (2002)
Oligomeric state and stoichiometry of p24 proteins in the early secretory pathway. J Biol Chem. 277(48):46504-11.Jenne N, Frey K, Brügger B, Wieland FT. (2002)
The Sur7p family defines novel cortical domains in Saccharomyces cerevisiae, affects sphingolipid metabolism, and is involved in sporulation. Mol Cell Biol. 22(3):927-34.Young ME, Karpova TS, Brügger B, Moschenross DM, Wang GK, Schneiter R, Wieland FT, Cooper JA. (2002)
Vesicular transport: the core machinery of COPI recruitment and budding. J Cell Sci. 115(Pt 16):3235-40. Review.Nickel W, Brügger B, Wieland FT. (2002)
Membrane Trafficking: How Transport Vesicles Fuse With Target Membranes in "Protein Modules in Cellular Signalling", eds Heilmeyer L. and Friedrich P. (IOS Press Amsterdam), pp. 257-261 (Review).Walter Nickel, Britta Brügger and Felix T. Wieland (2001)
Molecular Mechanisms of COPI Vesicle Formation in "Protein Modules in Cellular Signalling", eds. Heilmeyer L. and Friedrich P. (IOS Press Amsterdam), pp. 262-267 (Review).Britta Brügger, Walter Nickel, and Felix T. Wieland (2001)
Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23. The EMBO journal, 20(23), 6751–6760. https://doi.org/10.1093/emboj/20.23.6751Gommel, D. U., Memon, A. R., Heiss, A., Lottspeich, F., Pfannstiel, J., Lechner, J., Reinhard, C., Helms, J. B., Nickel, W., & Wieland, F. T. (2001)
Sphingomyelin-enriched microdomains at the Golgi complex. Mol Biol Cell 12(6):1819-33.Gkantiragas I, Brügger B, Stüven E, Kaloyanova D, Li XY, Löhr K, Lottspeich F, Wieland FT, Helms JB. (2001)
Evidence for segregation of sphingomyelin and cholesterol during formation of COPI-coated vesicles. J Cell Biol. 151(3):507-18.Brügger B, Sandhoff R, Wegehingel S, Gorgas K, Malsam J, Helms JB, Lehmann WD, Nickel W, Wieland FT. (2000)
Receptor-Dependent Formation of COPI-Coated Vesicles From Chemically Defined Donor Liposomes. Methods Enzymol. 329:388-404.Walter Nickel and Felix T. Wieland (2000)
A role for ADP-ribosylation factor in the control of cargo uptake during COPI-coated vesicle biogenesis. FEBS Lett. 462:267-272.Jörg Malsam, Daniel Gommel, Felix. T. Wieland, and Walter Nickel (1999)
Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors. Cell 96, 495-506.M. Bremser, W. Nickel, M. Schweikert, M. Ravazzola, M. Amherd, C.A. Hughes, T.H. Söllner, J. E. Rothman, and F. T. Wieland (1999)
Determination of cholesterol at the low picomole level by nano-electrospray ionization tandem mass spectrometry. J Lipid Res. 40(1):126-32.Sandhoff R, Brügger B, Jeckel D, Lehmann WD, Wieland FT. (1999)
Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane. J Cell Biol. 146(4):741-54.Schneiter R, Brügger B, Sandhoff R, Zellnig G, Leber A, Lampl M, Athenstaedt K, Hrastnik C, Eder S, Daum G, Paltauf F, Wieland FT, Kohlwein SD. (1999)
GTP-dependent Binding of ARF to Coatomer in Close Proximity to the Binding Site for Dilysine Retrieval Motifs and p23. J. Biol. Chem. 274, 14198-14203.L. Zhao, B. Helms, J. Brunner, and F. Wieland (1999)
Maturation of the axonal plasma membrane requires upregulation of sphingomyelin synthesis and formation of protein-lipid complexes. EMBO J. 18(7):1761-71.Ledesma MD, Brügger B, Bünning C, Wieland FT, Dotti CG. (1999)
Mechanisms of vesicle formation: insights from the COP system. Curr. Opin. Cell Biol. 11(4), 440-446.Wieland, F., & Harter, C. (1999)
p24 and p23, the major transmem-brane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway. FEBS Lett. 447:179-185.D. Gommel, L. Orci, E.M. Emig, M.J. Hannah, M. Ravazzola, W. Nickel, J.B. Helms, F.T. Wieland and K. Sohn (1999)
Receptor induced polymerisation of coatomer. Proc. Natl. Acad. Sci U.S.A. 96, 1224-1228.C. Reinhard, C. Harter, M. Bremser, B. Brügger, K. Sohn. B. Helms, and F. Wieland (1999)
A putative heterotrimeric G protein inhibits the fusion of COPI-coated vesicles. Segregation of heterotrimeric G proteins from COPI-coated vesicles. J Biol Chem. 273(24):15203-8.Helms JB, Helms-Brons D, Brügger B, Gkantiragas I, Eberle H, Nickel W, Nürnberg B, Gerdes HH, Wieland FT. (1998)
A single binding site for di-lysine motifs and p23 within the g-subunit of coatomer. Proc. Natl. Acad. Sci. USA. 95,11649-11654C. Harter, and F. Wieland (1998)
Biosynthetic Protein Transport Through the Early Secretory Pathway. Histochem. Cell Biol. 109:477-486 (Review).Walter Nickel and Felix T. Wieland (1998)
Functional organization of the Golgi apparatus in glycosphingolipid biosynthesis. Lactosylceramide and subsequent glycosphingolipids are formed in the lumen of the late Golgi. J.Biol.Chem. 273:2939-2946.Lannert, H., K. Gorgas, I. Meissner, F.T. Wieland, and D. Jeckel (1998)
Intracellular Transport of Glycoproteins and Glycolipids. Trends Glycosci. Glycotechnolog. 10:1-11 (Review).Walter Nickel, Britta Brügger, and Felix T. Wieland (1998)
Peroxisome biogenesis: involvement of ARF and coatomer. J. Cell Biol. 141, 373-383.M. Passreiter, M. Anton, D. Lay, R. Frank, C. Harter, F.T. Wieland, K. Gorgas and W.W. Just (1998)
Protein and lipid sorting between the endoplasmic reticulum and the Golgi complex. Semin Cell Dev Biol. 9(5):493-501. Review.Nickel W, Brügger B, Wieland FT. (1998)
Reversible dissociation of coatomer: Functional characterization of a b/d-coat protein subcomplex. Proc. Natl. Acad. Sci. U.S.A., 95, 2140-2145.J. Pavel, C. Harter, and F. Wieland (1998)
Uptake by COPI-coated vesicles of both anterograde and retrograde cargo is inhibited by GTPgammaS in vitro. J. Cell Sci. 111:3081-3090.Walter Nickel, Jörg Malsam, Karin Gorgas, Mariella Ravazzola, Nicole Jenne, J. Bernd Helms, and Felix T. Wieland (1998)
Biogenesis of COPI-Coated Transport Vesicles. FEBS Lett. 413:395-400 (Review).Walter Nickel and Felix T. Wieland (1997)
Direct and GTP-dependent interaction of ADP ribosylation factor 1 with coatomer subunit beta. Proc Natl Acad Sci USA 94(9):4418-23.Zhao L, Helms JB, Brügger B, Harter C, Martoglio B, Graf R, Brunner J, Wieland FT. (1997)
p23, a major COPI-vesicle membrane protein, constitutively cycles through the early secretory pathway. Proc. Natl. Acad. Sci. U.S.A. 94, 11393-11398.W. Nickel, K. Sohn, C. Bünning, and F. Wieland (1997)
Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. Proc Natl Acad Sci USA 94(6):2339-44.Brügger B, Erben G, Sandhoff R, Wieland FT, Lehmann WD. (1997)
A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding. J. Cell Biol. 135, 1238-1248K. Sohn, L. Orci, M. Ravazzola, M. Amherdt, M. Bremser, F. Lottspeich, K. Fiedler, J. B. Helms, and F. Wieland (1996)
Architecture of coatomer: molecular characterization of d-COP and protein interactions within the complex. J. Cell Biol. 135, 53-61.D. Faulstich, S. Auerbach, L. Orci, M. Ravazzola, S. Wegehingel, F. Lottspeich, G. Stenbeck, C. Harter, F. T. Wieland, and H. Tschochner (1996)
Forskolin stimulates detoxification of brefeldin A. J. Biol. Chem. 271:15870-15873.Walter Nickel, J. Bernd Helms, Richard E. Kneusel, and Felix T. Wieland (1996)
Non-clathrin-coat protein g, a subunit of coatomer, binds to the cytoplasmic dilysine motif of membrane proteins of the early secretory pathway. Proc. Natl. Acad. Sci. U.S.A. 93, 1902-1906C. Harter, J. Pavel, F. Coccia, E. Draken, S. Wegehingel, H. Tschochner, and F. Wieland (1996)
Protein sorting by transport vesicles. Science 272, 227-234.*J. E. Rothman and F. T. Wieland (1996)
Non-clathrin-coat protein a is a conserved subunit of coatomer and in Saccharomyces cerevisiae is essential for growth. Proc. Natl. Acad. Sci. U.S.A. 92, 3229-3233.B. Gerich, L. Orci, H. Tschochner, F. Lottspeich, M. Ravazzola, M. Amherdt, F. Wieland, and C. Harter (1995)
VIP21/caveolin is a cholesterol-binding protein. Proc. Natl. Acad. Sci. U.S.A. 92, 10339-10343M. Murata, J. Peränen, R. Schreiner, F. Wieland, T. V. Kurzchalia, and K. Simons (1995)
Analysis of bacterial glycoproteins. Methods Mol Biol 14:119-29.Lechner J, Wieland F. (1993)
b'-COP, a Novel Subunit of Coatomer. EMBO J. 12, 2841-2845G. Stenbeck, C. Harter, A. Brecht, D. Herrmann, F. Lottspeich, L. Orci, and F. T. Wieland (1993)
Membrane topology of the 22 kDa integral peroxisomal membrane protein. FEBS Lett. 315(3), 217-22K.Kaldi,P. Diestelkotter,G. Stenbeck,S. Auerbach,U. Jakle,H.J. Magert,F.T. Wieland and W.W. Just (1993)
Glucosylceramide is synthesized at the cytosolic surface of various Golgi subfractions. J. Cell Biol. 117, 259-267D. Jeckel, A. Karrenbauer, K. N. J. Burger, G. van Meer, and F. Wieland (1992)
A Coat Subunit of Golgi-Derived non-Clathrin Coated Vesicles with Homology to the Clathrin Coated Vesicle Coat Protein b-Adaptin. Nature 349, 215-220.T. Serafini, G. Stenbeck, A. Brecht, F. Lottspeich, L. Orci, J. E. Rothman, and F. Wieland (1991)
The Rate of Bulk Flow from the Golgi to the Plasma Membrane. Cell 63, 259-267.A. Karrenbauer, D. Jeckel. W. Just, R. Birk, R. R. Schmidt, J. E. Rothman, and F. Wieland (1990)
Purification of an N-Ethylmaleimide-Sensitive Protein Catalyzing Vesicular Transport. Proc. Natl. Acad. Sci.U.S.A. 85, 7852-7856.M. R. Block, B. S. Glick, C. A. Wilcox, F. Wieland, and J. Rothman (1988)
The Rate of Bulk Flow from the Endoplasmic Reticulum to the Cell Surface. Cell 50, 289-300.F. Wieland, M. Gleason, T. Serafini, J. Rothman (1987)
Asparaginyl-N-Acetylgalactosamine: Linkage Unit of Halobacterial Glycosaminoglycan. J. Biol. Chem. 261, 1020-1024.G. Paul, F. Lottspeich, and F. Wieland (1986)
Iduronic acid is a constituent of sulfated dolichyl phosphate oligosaccharides in halobacteria. FEBS Lett. 195:77-81.Wieland F, Lechner J, Sumper M. (1986)
Sulfated Dolicholphosphate Oligosaccharides are Transiently Methylated during Biosynthesis of Halobacterial Glycoproteins. System. Appl. Microbiol. 7:286-392.Wieland F, Lechner J, Sumper M. (1986)
Biosynthesis of sulfated saccharides N-glycosidically linked to the protein via glucose. Purification and identification of sulfated dolichyl monophosphoryl tetrasaccharides from halobacteria. J Biol Chem 260:860-6.Lechner J, Wieland F, Sumper M. (1985)
Transient methylation of dolichyl oligosaccharides is an obligatory step in halobacterial sulfated glycoprotein biosynthesis. J Biol Chem 260:8984-9.Lechner J, Wieland F, Sumper M. (1985)
Asparaginyl-Glucose: Novel Type of Carbohydrate Linkage. Proc. Natl. Acad. Sci. U.S.A 80, 5470-5474F. Wieland, R. Heitzer, and W. Schäfer (1983)
The Cell Wall Glycoprotein of Halobacteria: Structural, Functional and Biosynthetiv Aspects. Zentralbl. Bacteriol. Mikrobiol. Hyg. 1 Abt. Orig. C 3,:161-170.Wieland F, Lechner J, Sumper M. (1982)
Sulfation of a repetitive saccharide in halobacterial cell wall glycoprotein. FEBS Lett 132:319-323.Wieland F, Lechner J, Bernhardt G, Sumper M. (1981)
Distribution of Yeast Fatty Acid Synthetase subunits: Threedimensional Model of the Enzyme. Proc. Natl. Acad. Sci. U.S.A. 75, 5792-5796.F. Wieland, E. A. Siess, L. Renner, C. Verfürth, and F. Lynen (1978)
CV
CURRICULUM VITAE
Education: Study of Chemistry at the University of Munich
Master's Degree in chemistry 1975
PhD at the Max-Planck-Institute for Biochemistry
in Martinsried/Munich with Feodor Lynen 1975 - 1978
Career: Wissenschaftlicher Assistent (time-limited position for research)
at the Max-Planck-Institute 1976 - 1978
Akademischer Rat at the University of Regensburg 1978 - 1984
Member of SFB 43 (Collaborative Research Program), Regensburg 1981 - 1988
"Biochemistry of membranes and cell surfaces".
Habilitation for Biochemistry, venia legendi 1984
Akademischer Oberrat at the University of Regensburg 1984 - 1986
Research award by the Deutsche Forschungsgemeinschaft 1986 - 1988
(Dept. of Biochemistry, Stanford University, CA., USA)
Full professor and director of the Institute for Biochemistry I
at the University of Heidelberg 1988 - 1997
Chairman of SFB 352 (collaborative research program) 1991 - 2003
Heidelberg: "Molecular mechanisms of intracellular
transport processes"
Dean of the faculty “Naturwissenschaftliche Medizin” 1991 - 1992
Member for Biochemistry of the search committee
“Preclinical Medicine” at the Charité, Humboldt University Berlin 1991 - 1992
President elect of the German Society for Biochemistry and
Molecular Biology (GBM) 2003 - 2005
President GBM 2005 - 2007
Founding Director of the Heidelberg Biochemistry-Centre (BZH) 1997 - 2003
Managing Editor FEBS Letters 2001 - present
Chairman of SFB 638 (collaborative research program) 2003 - present
Heidelberg: ”Dynamics of macromolecular complexes
in biosynthetic transport”
Awards: Research award from the German Research Council 1986 - 1988
Honorary member of the Charité, Humboldt University Berlin 1993
Elected member of EMBO 2000
Heinrich Wieland Prize 2001
Elected Member of the Deutsche Akademie der Naturforscher 2003
Leopoldina (German National Academy of Sciences)
Feldberg Foundation Prize 2006
Elected Member of the Academia Europea 2011
Lab Members
Contact
Heidelberg University
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg
Office:
+49 6221 54-4150 Fax:
+49 6221 54-4366 E-Mail:
felix.wieland@bzh.uni-heidelberg.de